pgsk3α β (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
pgsk3α β
Pgsk3α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 842 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgsk3%CE%B1+%CE%B2/Phospho-GSK-3alpha%2Fbeta+(Ser21%2F9)+Antibody/pm41639460-653-107-108
Average 96 stars, based on 842 article reviews
Pgsk3α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 842 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgsk3%CE%B1+%CE%B2/Phospho-GSK-3alpha%2Fbeta+(Ser21%2F9)+Antibody/pm41639460-653-107-108
Average 96 stars, based on 842 article reviews
pgsk3α β - by Bioz Stars,
2026-09
96/100 stars
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Incubation:Article Title: Employing splice-switching oligonucleotides and AAVrh74.U7 snRNA to target insulin receptor splicing and cancer hallmarks in osteosarcoma Article Snippet: .. 620 Membranes were blocked for 30 minutes in casein buffer (Thermo Scientific, Cat# 37582, 621 Waltham, MA) and incubated overnight at 4°C in the following antibodies: pAKT (Ser473) 622 (1:1000, Cell Signaling, Cat# 4060S, clone D9E, Danvers, MA), panAKT (1:1000, Cell Signaling, 623 Cat# 4691S, clone C67E7, Danvers, MA), Article Title: Employing splice-switching oligonucleotides and AAVrh74.U7 snRNA to target insulin receptor splicing and cancer hallmarks in osteosarcoma Article Snippet: Protein concentrations were calculated using the Pierce BCA Protein Assay Kit (Thermo Scientific, Cat# 23225), and approximately 30 μg protein was loaded onto a denaturing NuPAGE 4 to 12% Bis-Tris gel (Invitrogen, Cat# NP0336BOX) with a Precision Plus protein standard (Bio-Rad, Cat# 1610376, Hercules, CA). .. Gels were run at 120 V for 105 min, and the separated proteins were transferred to polyvinylidene fluoride membranes on ice at 35 V for 60 min. Membranes were blocked for 30 min in casein buffer (Thermo Scientific, Cat# 37582) and incubated overnight at 4°C in the following antibodies: pAKT (Ser473) (1:1,000, Cell Signaling, Cat# 4060S, clone D9E), panAKT (1:1,000, Cell Signaling, Cat# 4691S, clone C67E7), Control:Article Title: In vivo genome‐editing screen identifies tumor suppressor genes that cooperate with Trp53 loss during mammary tumorigenesis Article Snippet: Lysates were separated by SDS/PAGE on 4‐20% Bis‐Tris pre‐cast gels (Invitrogen) and transferred onto polyvinylidene difluoride membrane (Millipore, Burlington, MA, USA). .. Membranes were probed with primary antibodies against AXIN1 (C76H11, Cell Signaling Technologies), pan‐AKT (C67E7, Cell Signaling Technologies), p‐AKT (D9E, Cell Signaling Technologies), GSK3α/β (D75D3, Cell Signaling Technologies), Binding Assay:Article Title: Confirmation of Decreased Rates of Cerebral Protein Synthesis In Vivo in a Mouse Model of Tuberous Sclerosis Complex Article Snippet: For normalization of Western blottings, we employed the Stain-Free technology (Bio-Rad) to normalize to total protein in the lane. .. Primary antibodies were diluted 1:1000 as follows: pAKT (protein kinase B) Ser 473 (Cell Signaling 4060), pAKT Thr 308 (Cell Signaling 4056), pAMPK (5′ adenosine monophosphate-activated protein kinase; Cell Signaling 2535), pCREB (cAMP response element binding protein; Cell Signaling 9198), peIF2α (eukaryotic translation initiation factor 2α; Cell Signaling 3398), pERK (extracellular regulated kinase; Cell Signaling 3370), Western Blot:Article Title: ZFTA-RELA ependymomas make itaconate to epigenetically drive fusion expression. Article Snippet: .. The following antibodies were used in the immunoblotting experiments: RELA (Cell Signaling Technology, 8242, 1:1,000); GAPDH (Cell Signaling Technology, 2118, 1:10,000); vinculin (Sigma Aldrich, V9264, 1:40,000); ACOD1-human (Abcam, ab222411, 1:1,000 and Novus Biologicals, NBP3-06244, 1:1,000); ACOD1-mouse (Cell Signaling Technology, 17805, 1:1,000); ZFTA (C11orf95) (VWR, 89379-010, AP11349B, 1:1,000); RFP (Abcam, Ab124754, 1:1,000); MAML3 (Invitrogen, PA5-13678, 1:1,000); SLC1A5 (Cell Signaling Technology, 5345, 1:1,000); GLS-human (Cell Signaling Technology, 49363, 1:1,000); GLS-mouse (Invitrogen, PA5-35365, 1:1,000); MYC (Abcam, 32072, 1:1,000); PTEN (Cell Signaling Technology, 9559, 1:1,000); pAKT (S473) (Cell Signaling Technology, 9271, 1:1,000); AKT (Cell Signaling Technology, 4056, 1:1,000); pS6RP (S235/236) (Cell Signaling Technology, 4858, 1:1,000); S6RP (Cell Signaling Technology, 2217, 1:1,000); Article Title: GPR109A mediates the effects of hippuric acid on regulating osteoclastogenesis and bone resorption in mice Article Snippet: The protein 187 lysates were quantified and separated by sodium dodecyl sulfate polyacrylamide gel 188 electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). .. This 189 was followed by immunoblotting with primary antibodies NFATc1 (SAB2101576, 190 Sigma-Aldrich, St. Louis, MO, USA), MMP9 (AB19016, Millipore, MA, USA), 191 Cathepsin K (ab187647,Abcam, MA, USA), GPR109A (#223312, US Biological, MA, 192 USA), β-catenin (BD Transduction Lab, #610154), Article Title: Lithium for schizophrenia: supporting evidence from a 12-year, nationwide health insurance database and from Akt1-deficient mouse and cellular models Article Snippet: .. After sample preparation, Western blotting was performed and the blot was probed with the following antibodies: Gsk3α/β (1:1000, #5676), Sample Prep:Article Title: Lithium for schizophrenia: supporting evidence from a 12-year, nationwide health insurance database and from Akt1-deficient mouse and cellular models Article Snippet: .. After sample preparation, Western blotting was performed and the blot was probed with the following antibodies: Gsk3α/β (1:1000, #5676), |
